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外泌体解决方案
678 人阅读发布时间：2020-10-27 17:11
外泌体（Exosome）是由细胞分泌而来的微小囊泡，直径约为 30-150 nm，天然存在于血液、尿液、唾液、母乳和细胞培养基等生物体液中，几乎所有类型的细胞都可以产生并释放外泌体[1]。目前，外泌体因其在细胞间通讯中的作用而被广泛地认识到，比如将不同生物分子(包括蛋白质、RNA和脂质)从一个细胞传递到另一个细胞[2]。越来越多的研究者开始对外泌体作为生物标志物在临床的应用、以及它的治疗用途进行研究和探索。Genecopoeia将为您的外泌体研究提供高质量的产品和服务。
【2】Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat. Cell Biol. 2007. 9, 654–659.
【3】Current Perspectives on In Vivo Noninvasive Tracking of Extracellular Vesicles with Molecular Imaging. BioMed Research International. 2017. 9158319, 11 pages.
【4】Bone marrow mesenchymal stem cell-derived exosome uptake and retrograde transport can occur at peripheral nerve endings, Artificial Cells, Nanomedicine, and Biotechnology. 2019. 47:1, 2918-2929.
【5】Cancer-associated fibroblast exosomes regulate survival and proliferation of pancreatic cancer cells. Oncogene. 2017. 36(13): 1770–1778.
【6】Exosomes and their roles in immune regulation and cancer. Semin Cell Dev Biol.2015. 40: 72-81.
【7】Investigation of Content, Stoichiometry and Transfer of miRNA from Human Neural Stem CellLine Derived Exosomes. PLoS One. 2016. 11: e0146353.
【8】The Biology of Cancer Exosomes: Insights and New Perspectives. Cancer Res. 2017. 77: 6480-6488.
【9】Identification of cell-free microRNAs in the urine of patients with prostate cancer. Urol Oncol. 2015. 33(1):16.e17-16.e22.
【10】MicroRNA-29c in urinary exosome/microvesicle as a biomarker of renal fibrosis. Am J Physiol Renal Physiol. 2013. 305(8):F1220-7.
【11】Protocol for serum exosomal miRNAs analysis in prostate cancer patients treated with radiotherapy. J Transl Med. 2018. 16(1):223.
【12】Exosome-Derived miR-130a Activates Angiogenesis in Gastric Cancer by Targeting C-MYB in Vascular Endothelial Cells. Mol Ther. 2018. 3;26(10):2466-2475.
【13】Tissue and exosomal miRNA editing in Non-Small Cell Lung Cancer. Sci Rep. 2018. 8(1):10222.
【14】Hypoxic BMSC-derived exosomal miRNAs promote metastasis of lung cancer cells via STAT3-induced EMT. Mol Cancer. 2019. 18(1):40.
【15】Downregulation of exosome-encapsulated miR-548c-5p is associated with poor prognosis in colorectal cancer. J Cell Biochem. 2018. Pages 1457-1463.
【16】Simultaneous and multiplexed detection of exosome microRNAs using molecular beacon. Biosens Bioelectron. 2016. 86:202-210.
【17】Protocol for serum exosomal miRNAs analysis in prostate cancer patients treated with radiotherapy. J Transl Med. 2018. 16(1):223.
外泌体预制慢病毒
Genecopoeia提供携带不同标记（tdTomato, mCherry-Flag, Flag-Gluc）的外泌体标志物CD9, CD63和CD81的预制慢病毒(图一)。可用于示踪外泌体的形成、分泌、靶向和运输机制[3,4,5]。另外，Genecopoeia还提供可用于动物活体实验研究的外泌体预制慢病毒。
图一. 外泌体示意图（Ibrahim A, Marbán E. Annual Review of Physiology, 2016）
外泌体预制miRNA检测阵列
外泌体是存在于循环中的纳米小泡，参与细胞之间的通讯和调节不同的生物过程。miRNAs是外泌体转运的重要组成部分，可作为潜在的生物标志物[6]。在不同细胞微环境刺激下，细胞能主动、特异地将miRNAs包裹，并通过外泌体的形式分泌到细胞外环境以响应不同的刺激，外泌体miRNAs转移到受体细胞以调节靶基因的表达[7]。外泌体miRNAs不仅有助于了解肿瘤发生的生物学机制，而且有助于进一步探索治疗途径[8]。 Genecopoeia通过对发表文献的全面检索，精心筛选miRNA与外泌体之间的密切关系，提供用于分析与外泌体相关的miRNA表达的miprofile™ exosome miRNA qPCR阵列。miProfile™外泌体miRNA阵列可以帮助研究人员分析不同样本(尿液[9,10]、血液[11,12]或牛奶)和各种癌症（如肺癌[13,14]、结直肠癌[15]、乳腺癌[16]、前列腺癌[17]等）的外源体中miRNA的差异表达，从而了解miRNA在不同细胞间相互通讯中的作用。
参考文献
【1】Exosomes. Annu. Rev. Biochem. 2019. 88:487–514.【2】Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat. Cell Biol. 2007. 9, 654–659.
【3】Current Perspectives on In Vivo Noninvasive Tracking of Extracellular Vesicles with Molecular Imaging. BioMed Research International. 2017. 9158319, 11 pages.
【4】Bone marrow mesenchymal stem cell-derived exosome uptake and retrograde transport can occur at peripheral nerve endings, Artificial Cells, Nanomedicine, and Biotechnology. 2019. 47:1, 2918-2929.
【5】Cancer-associated fibroblast exosomes regulate survival and proliferation of pancreatic cancer cells. Oncogene. 2017. 36(13): 1770–1778.
【6】Exosomes and their roles in immune regulation and cancer. Semin Cell Dev Biol.2015. 40: 72-81.
【7】Investigation of Content, Stoichiometry and Transfer of miRNA from Human Neural Stem CellLine Derived Exosomes. PLoS One. 2016. 11: e0146353.
【8】The Biology of Cancer Exosomes: Insights and New Perspectives. Cancer Res. 2017. 77: 6480-6488.
【9】Identification of cell-free microRNAs in the urine of patients with prostate cancer. Urol Oncol. 2015. 33(1):16.e17-16.e22.
【10】MicroRNA-29c in urinary exosome/microvesicle as a biomarker of renal fibrosis. Am J Physiol Renal Physiol. 2013. 305(8):F1220-7.
【11】Protocol for serum exosomal miRNAs analysis in prostate cancer patients treated with radiotherapy. J Transl Med. 2018. 16(1):223.
【12】Exosome-Derived miR-130a Activates Angiogenesis in Gastric Cancer by Targeting C-MYB in Vascular Endothelial Cells. Mol Ther. 2018. 3;26(10):2466-2475.
【13】Tissue and exosomal miRNA editing in Non-Small Cell Lung Cancer. Sci Rep. 2018. 8(1):10222.
【14】Hypoxic BMSC-derived exosomal miRNAs promote metastasis of lung cancer cells via STAT3-induced EMT. Mol Cancer. 2019. 18(1):40.
【15】Downregulation of exosome-encapsulated miR-548c-5p is associated with poor prognosis in colorectal cancer. J Cell Biochem. 2018. Pages 1457-1463.
【16】Simultaneous and multiplexed detection of exosome microRNAs using molecular beacon. Biosens Bioelectron. 2016. 86:202-210.
【17】Protocol for serum exosomal miRNAs analysis in prostate cancer patients treated with radiotherapy. J Transl Med. 2018. 16(1):223.
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